Fasciola gigantica of the most important helminths in the liver and biliary tract of domestic animals, causes widespread lesions in ruminants living in southwestern of Iran. Acording to reports of the occurrence of leiomyoma in infected cows with fasciola gigantica, as well as information on the increase in the amount of polyamines due to hepatic lesions, and also changes in the levels of enzymes involved in metabolism of these compounds in cancers, the study has been designed to investigate the relation between lesions of infected livers Fasciola gigantica and changes of polyamines in cows.
50 livers infected with fasciola gigantica, along with their blood samples were collected from Ahvaz slaughterhouse for histopathology, biochemical, immunologic and hematological tests. Also 15 non-infected livers were collected from Yasuj slaughterhouse. Routine and specific histopathologic studies were performed on prepared tissue samples and the expression of α-SMA and desmin indicators was evaluated using indirect immuno-peroxidase method. Amount of polyamines were measured using high performance liquid chromatography. Changes in IL-4, TNF-α and hematology profile were evaluated by DOT-ELIZA and CBC tests, respectively.
Leiomyoma was identified as the most important lesion in 90% of the infected livers. Also, uniform and potent expression of α-SMA against non uniform and weak to moderate expression of desmin was observed in livers with leiomyoma and fasciola infection. Statistical T-test showed a significant alterations in biogenic amines of putrescine (P=0.02) and spermine (P=0.01), as well as the hematological parameters of RBC (P=0.02) and MCHC (P=0.00) between infected and noninfected livers.
The results of the present study showed the amount of polyamines may increase due to the lesions of fasciola gigantica and influence of biosynthetic and catabolic enzymes and proteinases. On the other hand, the increase in polyamines may contribute to the induction, progress and development of lieomyoma observed in the liver.
 

Ovine pulmonary adenocarcinoma (OPA) is a contagious lung cancer of sheep caused by jaagsiekte sheep retrovirus (JSRV) which inducing acinary and papillary proliferation and transformation in alveolar and brochiolar secretary epithelial cells, involve in pathophysiology of disease. Considering the contagious pattern of OPA and the importance of its earlier recognition, beyond the histopathological examinations, this project also tried to identify the prevalence of JSRV amongst sheep slaughtered in city of Ahvaz in Iran. For this purpose, the lungs of 176 sheep which sampled randomly, in addition to12 lungs grossly suspected to OPA lesions were collected from Ahvaz Sloughtherhouse during February through May 2011. Sections from 4 different lobes of each lung and associated mediastinal lymph nodes and tumor suspected areas were subjected to the histopathological examinations. Consistently, semi nested PCR for detection of JSRV proviral DNA were also performed on the DNA extracted from the Lungs and mediastinal lymph nodes. Of 176 randomly selected samples, 11 samples demonstrated Histopathological signs of ovine pulmonary adenocarcinoma (6/25%) while applying PCR experiment, 54 animals (30/68%) were detected with proviral DNA of jaagsiekte sheep retrovirus. All 12 tumor-suspected samples were also identified with adenocarcinoma lesions confirmed by histopathological examination. Altogether, 16 animals were detected with the macroscopic sings of tumor with the different pathological forms of pulmonary adenocarcinoma including classical, atypical and combinational forms which were distinctively described. Noteworthy none of samples showed metastatic lesions in lymph nodes. Concurrent pathological and molecular examination also demonstrated that JSRV positive animals had more susceptibility to grow other pulmonary lesions such as bronchopneumonia and interstitial pneumonia than JSRV negative one. Taken together, the results of present study was in favor of a high prevalence of ovine pulmonary adenocarcinoma which was also associated with a higher prevalence of JSRV infection of sheep in Ahvaz, the observation that highlights the importance of JSRV screening methods development to predict preclinical condition.